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KMID : 1024520100190111391
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Journal of the Environmental Sciences
2010 Volume.19 No. 11 p.1391 ~ p.1396
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Isolation and identification of a Biphenyl-degrading bacterium, Pseudomonas sp. Ds-94
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Lee Dae-Sung
Jeong Seong-Yun
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Abstract
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Three biphenyl-degrading microorganisms were isolated from polluted soil samples in Sasang-gu, Busan. Among them, isolate DS-94 showing the strong degrading activity was selected. The morphological, physiological, and biochemical characteristics of DS-94 were investigated by API 20NE and other tests. This bacterium was identified as the genus Pseudomonas by 16S rDNA sequencing and designated as Pseudomonas sp. DS-94. The optimum temperature and pH for the growth of Pseudomonas sp. DS-94 were 25℃ and pH 7.0, respectively. This isolate could utilize biphenyl as sole source of carbon and energy. Biphenyl-degrading efficiency of this isolate was measured by HPLC analysis. As a result of biological biphenyl-degradation at high biphenyl concentration (500 mg/L), biphenyl-removal efficiency by this isolate was 73.5% for 7 days.
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KEYWORD
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Biphenyl-degrading activity, Contaminated soil, Pseudomonas sp. Ds-94
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